人主動(dòng)脈血管平滑肌細(xì)胞說(shuō)明書(shū)

【資料簡(jiǎn)介】

T/G HA-VSMC(人主動(dòng)脈血管平滑肌細(xì)胞)  
 
Cell Biology 
ATCC® Number: CRL-1999™       Price: 
Designations: T/G HA-VSMC  Depositors: RR Gifford 
Biosafety Level: 1 Shipped: frozen 
Medium & Serum: See Propagation Growth Properties: adherent 
Organism: Homo sapiens (human) Morphology: fibroblast
 
 
Source: Organ: aorta
Tissue: smooth muscle
Disease: normal 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
Related Cell Culture Products 
Isolation: (The T/G HA-VSMC line was established from the normal aorta of an 11 month old child.) 
Applications: transfection host (technology from amaxa) 
DNA Profile
(STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12
D5S818: 12,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 16 
Cytogenetic
Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal. 
Age: 11 months 
Gender: female 
Ethnicity: Caucasian 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.
Temperature: 37.0C 
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.
 
Subc*tion ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
 
Medium renewal: Every 2 to 3 days
 
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature