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Anti-Bacillus anthracis lethal factor抗體,炭疽桿菌致死因子LF抗體
英文介紹:The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-Bacillus anthracis lethal factor抗體,炭疽桿菌致死因子LF抗體抗體溶解方法及分裝:
1、購買抗體后,查看標(biāo)簽上的規(guī)格,如:0.1ml、0.2ml
2、以規(guī)格為 0.1ml 的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加 0.1ml 雙蒸水到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加與標(biāo)簽上規(guī)格*的(如:0.2ml)雙蒸水溶解,
混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規(guī)格的 50%)雙蒸水到試管內(nèi),再加 0.05ml(規(guī)格的 50%)
的甘油到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加標(biāo)簽上規(guī)格的 50%(如:0.1ml)雙蒸水,再加
標(biāo)簽上規(guī)格的 50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
抗體溶解后,將其分裝成 5-10 支(以 0.1ml 抗體為例,20ulx5 支或 10ulx10 支), -20℃保存,用
一支取一支,避免反復(fù)凍溶。
另:預(yù)試驗可做幾個梯度 1:100、1:200、1:400 稀釋,如果背景高,還可再稀釋,選一個zui
佳的稀釋比例,再正式做,效果。
產(chǎn)品名稱:Anti-Bacillus anthracis lethal factor抗體,炭疽桿菌致死因子LF抗體
產(chǎn)品編號:BYK-12564R
英文名稱:Anti-Bacillus anthracis lethal factor
中文名稱:炭疽桿菌致死因子LF抗體
英文別名:Anthrax lethal factor; Anthrax lethal toxin endopeptidase component; Anthrax LF; bacillus anthracis lethal factor; Lef; LF; LEF_BACAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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